| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 331 | Brachyspira pilosicoli | GTGCTGGTGCTATGGGGTT | AGCAACAGAAACTGAAGCAGC | 60.00 | 59.66 | 281 | 53 |
100.00%
|
100.00%
|
| Primer 332 | Brachyspira pilosicoli | GGAAGTGCTTGCGACAGTG | CTCCCTTGTCCCCAAAACCT | 59.43 | 59.52 | 253 | 53 |
100.00%
|
100.00%
|
| Primer 333 | Brachyspira pilosicoli | GGAAGTGCTTGCGACAGTG | TCCCTTGTCCCCAAAACCT | 59.43 | 58.35 | 252 | 53 |
100.00%
|
100.00%
|
| Primer 334 | Brachyspira pilosicoli | GCTGATGACGGTACTACTGGA | AACCAACCGCTAGCTCCT | 58.98 | 58.19 | 218 | 53 |
100.00%
|
100.00%
|
| Primer 335 | Brachyspira pilosicoli | ACCTGGTGCTGCTACTCCT | AGCCAAGTACCTCCGCAT | 60.23 | 58.27 | 228 | 53 |
100.00%
|
50.00%
|
| Primer 336 | Brachyspira pilosicoli | ACCTGGTGCTGCTACTCCT | AAAGCCAAGTACCTCCGCA | 60.23 | 59.24 | 230 | 53 |
100.00%
|
50.00%
|
| Primer 337 | Brachyspira pilosicoli | GTTGAAGCTTACGGCAGCAG | GCAATCTTAGCTTCTTCGCCT | 59.83 | 58.72 | 257 | 53 |
100.00%
|
100.00%
|
| Primer 338 | Brachyspira pilosicoli | GTTGAAGCTTACGGCAGCA | GCAATCTTAGCTTCTTCGCCT | 58.76 | 58.72 | 257 | 53 |
100.00%
|
100.00%
|
| Primer 339 | Brachyspira pilosicoli | TTGAAGCTTACGGCAGCAGT | GCAATCTTAGCTTCTTCGCCT | 60.25 | 58.72 | 256 | 53 |
100.00%
|
100.00%
|
| Primer 340 | Brachyspira pilosicoli | AGCGGAATAGACGGCTCAC | ACTTCATCGCGTACTGGCAA | 59.56 | 60.04 | 203 | 53 |
100.00%
|
100.00%
|